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目的研究成年山羊精子在附睾成熟过程中与凝集素芯片结合的差异。方法精子分别取自成年山羊附睾头、体、尾部,经2%多聚甲醛/0.2%戊二醛固定后,然后进行碘化丙啶染色标记,再分别与含有91种凝集素的芯片结合。信号经提取后,利用SPSS16.0和R语言对其进行后续分析和比较。结果山羊附睾头、体、尾各部分精子与含各类型糖基特异性的凝集素芯片结合分析后发现,与山羊附睾头部精子结合的凝集素有40种,体部精子结合的有48种,尾部精子结合的有42种。来自附睾不同区段的精子与凝集素结合强度也有显著差异,其中,甘露糖特异性的凝集素与体部精子结合最高,复杂结构特异性的凝集素与附睾头、体、尾各部分精子的结合强度呈梯度下降趋势。结论山羊精子膜表面的糖被,随着精子在附睾中的成熟,也在不断地发生变化的。
Objective To study the differences between adult goat sperm and lectin chips during epididymal maturation. Methods The sperms were taken from the head, tail and body of the epididymal goat respectively, fixed with 2% paraformaldehyde / 0.2% glutaraldehyde, then stained with propidium iodide, and then combined with chips containing 91 kinds of lectins respectively. The signal was extracted, the use of SPSS16.0 and R language for its subsequent analysis and comparison. Results The results showed that 40 spermatozoites that bind to spermatozoa of goat epididymis and 48 spermatozoa that bind to body sperm There are 42 tail sperm binding. There was also a significant difference in the binding strength between sperm and lectin from different segments of epididymis. Among them, mannose-specific lectin had the highest binding to body sperm, complex structure-specific lectin and spermatozoa The bonding strength shows a gradient decreasing trend. Conclusion Goat sperm membrane sugar on the surface, with the sperm in the epididymis mature, but also constantly changing.