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以小麦 -中间偃麦草附加系 L1为抗源 ,选育出抗黄矮病小麦异源易位系 HW6 4 2 ,YW4 4 3,YW2 4 3,Y9910 2 9等。本文以上述易位系及其抗源、小麦亲本作供试材料 ,鉴定出一个微卫星 (Simple sequence repeat,SSR)标记gwm37- 450 ,可跟踪、检测源于 L1的抗黄矮病基因。将难以分辨、稳定性差的 gwm37- 450 特异带分离、克隆、测序 ,根据测序结果重新设计 1对特异 PCR引物 ,转化为扩增产物有无的 SCAR(sequence characterizied amplified region)标记SC- W374 50 。利用 HW6 4 2 /中 86 0 1的 F2 群体 ,对 gwm 37- 450 和 SC- W374 50 进行分析 ,结果表明 ,二者均与抗黄矮病基因紧密连锁 ,后者较前者稳定。利用 SC- W374 50 跟踪抗黄矮病基因 ,将抗黄矮病基因、抗白粉病基因向优良小麦品种中麦 16、宛 710 7转育 ,快速选育出兼抗黄矮病、白粉病的回交植株 2 7个。实践了分子标记辅助抗黄矮病小麦育种的技术路线。
The heterotic lines HW6 4 2, YW4 4 3, YW2 4 3, Y9910 2 9 and so forth were bred from wheat-Thinopyrum intermedium L1 as the resistant source. In this study, we constructed a simple sequence repeat (SSR) marker gwm37- 450 using the above translocation lines and their parents and wheat parents as the test materials, which can trace and detect the anti-yellow dwarf virus genes derived from L1. According to the results of sequencing, a pair of specific PCR primers was transformed into SCAR (sequence characterizied amplified region) marker SC-W37450 with or without amplification, and the gwm37-450 specific bands with poor stability and stability were isolated, cloned and sequenced. Analysis of gwm 37-450 and SC-W374 50 using the F2 population of HW6 4 2/86 0 1 showed that both of them were closely linked to the resistance gene to yellow dwarf virus, the latter being more stable than the former. Using SC-W37450 to trace anti-yellowish dwarf virus genes, the genes of resistance to yellowish dwarf and resistance to powdery mildew were transferred to wheat cultivar Zhongmai16 and Wan 7107, Backcrossing 27 plants. The practice of molecular marker-assisted anti-yellow dwarf wheat breeding technology route.