论文部分内容阅读
目的探讨不同照射剂量对小鼠淋巴细胞的增殖及杀伤能力的影响,探讨经照射、非经照射Parent→F1,F1→F1半相合免疫细胞输注小鼠模型嵌合情况及抗肿瘤效应。方法制备经照射的不同浓度的BALB/c、CC3HF1(BALB/c×C3H)小鼠脾细胞悬液,用MTT法、LDH法检测不同剂量照射对细胞增殖能力、杀伤能力的影响。以荷Hepa1-6瘤小鼠CB6F1(H-2b/d)作为受者鼠,以雌性BALB/c,BALB/c×C3H小鼠为半相合供者鼠,用流式方法检测小鼠的嵌合状态。种瘤后每3天游标卡尺测量肿瘤大小并计算肿瘤体积。取荷瘤小鼠肝、肠、皮肤、脾脏做组织病理切片,并进行HE染色,检测有无GvHD产生。结果 5Gy照射可以显著抑制小鼠淋巴细胞的增殖,且对淋巴细胞的杀伤活性影响不大。Parent→F1输注模型中,供者淋巴细胞在小鼠体内出现先减少后增多,又减少的趋势。Parent→F1、F1→F1输注模型可诱发抗肿瘤效应,未出现GvHD。结论 Parent→F1,F1→F1半相合免疫细胞输注小鼠模型,具备可诱发抗肿瘤作用,同时照射可以抑制GvHD产生,是一种有效的小鼠抗肿瘤模型。F1→F1半相合免疫细胞输注小鼠模型是一种更接近临床应用的实验模型。
Objective To investigate the effects of different doses of radiation on the proliferation and cytotoxicity of mouse lymphocytes and to explore the chimerism and anti-tumor effects of irradiated mice irradiated with Parent → F1 and F1 → F1 haploidentical immune cells. Methods The splenocytes of BALB / c, CC3HF1 (BALB / c × C3H) mice were irradiated with different concentrations. The effects of different doses of irradiation on proliferation and cytotoxicity were detected by MTT assay and LDH assay. Female Hepatoma BALB / c, BALB / c × C3H mice were used as donor mice with Hepa1-6 tumor-bearing mice CB6F1 (H-2b / d) as recipients and flow cytometry Combined state. Tumor size was measured and the tumor volume calculated every 3 days after inoculation. Tumor-bearing mice liver, intestine, skin, spleen tissue histopathology, and HE staining to detect the presence or absence of GvHD. Results 5Gy irradiation could significantly inhibit the proliferation of mouse lymphocytes, and had little effect on the cytotoxic activity of lymphocytes. In the Parent → F1 infusion model, the number of donor lymphocytes decreased first, then increased, and then decreased in mice. Parent → F1, F1 → F1 infusion model can induce anti-tumor effect, did not appear GvHD. Conclusions It is an effective anti-tumor model in mice that Parent-F1 and F1-F1 haploidentical immune cells are infused into mice model to induce anti-tumor effect while irradiation can inhibit GvHD production. F1 → F1 haploidentical immune cell infusion mouse model is an experimental model closer to clinical application.