为了能在理想的条件下,研究诸多因素对肾功能的影响,人们一直试图发展一种离体肾的研究方法。自从1959年第一篇大鼠离体肾灌注(isolated perfused kidney, IPK)论文发表以来,经过30年的技术改进,使之逐渐成为研究肾脏功能的一个重要实验手段。IPK技术并不复杂,而且无血压、二氧化碳分压、神经和激素等因素的影响,并且 In order to study the effects of many factors on renal function under ideal conditions, people have been trying to develop a method of studying isolated kidneys. Since the publication of the first isolated perfused kidney (IPK) paper in 1959, 30 years of technological improvement have made it an important experimental tool for studying renal function. IPK technology is not complicated, and no blood pressure, partial pressure of carbon dioxide, nerves and hormones and other factors, and