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为选育耐高温耐盐红螺菌用于其粉状制剂研制,以降解水产养殖水体亚硝基氮功能达90%以上的耐盐红螺菌与能在60～70℃下生长的嗜热脂肪芽孢杆菌为出发菌株,通过正交试验探讨2种菌株的原生质体融合条件。分别对融合子进行温度、生长盐度和净化水质等功能测定,检出性能较优的融合子,同时采用电镜、细胞色素吸收光谱等方法,将融合子优良株与2种亲株进行比较鉴别。实验结果表明,2种菌株原生质体融合的最优条件为温度37℃,作用时间3 h,聚乙二醇(PEG)浓度为40%。在该优化条件下原生质体融合率为1.536%,筛选获得3株能在30～50℃良好生长的融合子(a、b、c)。融合子生长耐受温度较耐盐红螺菌亲株提高66%,但耐受盐度、降解养殖水体氨氮与亚硝基氮功能均与耐盐红螺菌亲株相近。其中融合子a菌株具有生长更快、性能更稳定的特点,其细胞形态特征、革兰氏染色属性、色素吸收峰等均与耐盐红螺菌相似。
To breed salt-tolerant Rhodospirides Rhizoctonia for the development of its powder formulations to reduce aquaculture water nitrosyl nitrogen function of Rhizoctonia solani and 90% can grow at 60 ~ 70 ℃ under the heat Bacillus stearothermophilus was the starting strain, and the protoplast fusion conditions of the two strains were discussed by orthogonal test. Fusants were tested for their functions such as temperature, growth salinity and water purification, respectively, and the fusants with better performance were detected. The fusion strains were compared with the two progenies by electron microscopy and cytochrome absorption spectroscopy . The experimental results showed that the optimal conditions for protoplast fusion of the two strains were 37 ° C for 3 h and 40% for polyethylene glycol (PEG). Under the optimized conditions, the protoplast fusion rate was 1.536%, and three strains (a, b, c) which could grow well at 30-50 ℃ were screened. The temperature of confluent growth was 66% higher than that of salt-tolerant Rhodobacter sp., But the tolerance to salinity and degradation of ammonia nitrogen and nitroso-nitrogen in aquaculture water were similar to that of salt-tolerant Rhodosporidium. Fusarium subfasciatus strain a with faster growth, more stable performance characteristics, and its cell morphology, Gram staining properties, pigment absorption peak Dengjun Rhizoctonia are similar.