紫树科植物喜树(Camptotheca acuminata)为中国本土产树木,其中含有一种天然抗癌生物碱喜树碱。喜树碱具有抑制拓扑异构酶Ⅰ的作用。目前,半合成的喜树碱模拟物已在美国市场作为抗癌药销售。由于化学合成的成本效益不理想,产品仍然依赖于天然喜树碱的供给。为鉴定高产量喜树碱生产线,本次开发了一种痕量测定喜树碱浓度的方法,能够对喜树进行快速筛选。 Camptotheca acuminata, a Chinese native tree, contains a natural anticancer alkaloid camptothecin. Camptothecin has the effect of inhibiting topoisomerase I. Currently, semisynthetic camptothecin mimetics have been marketed as anti-cancer drugs in the U.S. market. Because the cost-effectiveness of chemical synthesis is not ideal, the product still relies on the supply of natural camptothecin. In order to identify high-yielding camptothecin production lines, a method for the determination of camptothecin in trace amounts was developed to rapidly screen Camptotheca acuminata.