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以不同百合种间杂交获得的子房为外植体,根据子房的发育程度,采用子房切片、胚珠或者小胚进行离体培养,观察子房切片、胚珠和小胚的萌发和生长情况,以期筛选出适合小胚、胚珠和子房切片萌发和生长的培养基。结果表明:适合子房切片培养的培养基为MS+NAA 0~0.3mg/L+蔗糖30 000mg/L;适合胚珠培养的培养基为1/2MS+BA 0~0.2mg/L+蔗糖30 000mg/L;适合小胚培养的培养基为1/2MS+BA 0~0.2mg/L+NAA 0~0.2mg/L+蔗糖30 000mg/L。
The ovary obtained from the cross between different lilies was used as explants. According to the degree of ovary development, ovary sections, ovules or small embryos were used for in vitro culture to observe the germination and growth of ovary slices, ovules and small embryos , In order to screen out suitable for small embryo, ovule and ovary section germination and growth medium. The results showed that medium suitable for ovary section culture consisted of MS + NAA 0 ~ 0.3mg / L + sucrose 30 000mg / L and medium suitable for ovule culture with 1 / 2MS + BA 0 ~ 0.2mg / L + sucrose 30 000mg / L ; Suitable for small embryo culture medium 1 / 2MS + BA 0 ~ 0.2mg / L + NAA 0 ~ 0.2mg / L + sucrose 30 000mg / L.