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目的探讨AgNOR检测在急性白血病分型诊断中的意义。方法采用Ploton改良的AgNOR技术,对124例急性白血病和40例正常人AgNOR进行检测。结果急性白血病AgNOR比正常人明显增高(急性白血病为4.68±0.98/核,正常人1.36±0.24/核),两者相比有显著性差异(P<0.01)。从急性白血病各亚型AgNOR检测结果排列顺序看,M7最高(AgNOR 7.82±0.28/核),M3最低(AgNOR 3.52±1.07/核)。结论 AgNOR的检测可作为急性白血病的分型、鉴别诊断和疗效观察的指标之一。
Objective To investigate the significance of AgNOR in the differential diagnosis of acute leukemia. Methods Ploton modified AgNOR technique was used to detect 124 cases of acute leukemia and 40 cases of normal AgNOR. Results AgNOR in acute leukemia was significantly higher than that in normal people (acute leukemia was 4.68 ± 0.98 / nucleus and normal person was 1.36 ± 0.24 / nucleus). There was a significant difference between the two groups (P <0.01). According to the sequence of AgNOR test results, the highest M7 (AgNOR 7.82 ± 0.28 / nuclear) and the lowest M3 (AgNOR 3.52 ± 1.07 / nuclear) were found. Conclusion The detection of AgNOR can be used as a marker of acute leukemia, differential diagnosis and curative effect observation.