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目的 从分子流行病学角度探讨北京及其他地区结核分枝杆菌 (结核菌 )的分布特征。方法 构建以IS6 110为基础的结核菌DNA指纹图谱 ,应用MINTS软件进行处理 ,并用 χ2 检验比较不同组别结核病人临床分离菌株成簇率的差别。结果 H37Rv、BCG两个标准菌株和 6 2例结核病人临床分离菌株IS 6 110DNA指纹结果与国外同类报道一致 ;其中 70 % (4 4/6 2 )的临床分离菌株IS6 110DNA指纹相似值在 1 0 0～ 0 6 5之间 ;分组统计结果显示 ,男性组与女性组成簇率之间差异有显著性(P <0 .0 5 ) ,其他各组之间差异无显著性 (P >0 .0 5 )。结论 DNA指纹技术对结核菌在株水平的鉴定具有特异、灵敏等优点 ,可应用于结核流行病学研究。研究表明 ,北京及其他地区结核菌临床分离菌株多数遗传关系较近 ,且在基因水平上相关程度较强 ;结核菌在男性人群中的传播频率可能较女性更高
Objective To investigate the distribution of Mycobacterium tuberculosis (TB) in Beijing and other regions from the perspective of molecular epidemiology. Methods The DNA fingerprinting of Mycobacterium tuberculosis based on IS6 110 was constructed and processed by MINTS software. The difference of clustering rate among clinical isolates from different groups was compared by χ2 test. Results The results of DNA fingerprinting of IS 6 110 from two standard strains of H37Rv and BCG and 62 from tuberculosis patients were consistent with those reported in foreign countries. The similarity of DNA fingerprinting of IS6 110 in 70% (4 4/6 2) isolates was 10 (P <0.05). The statistical results of grouping showed that there was significant difference (P <0.05) between the clustering rate of males and females, but there was no significant difference among the other groups (P> 0.05) 5). Conclusion DNA fingerprinting has the advantages of specificity and sensitivity for the identification of Mycobacterium tuberculosis at the strain level and can be applied to the epidemiological study of tuberculosis. Studies have shown that most of the clinically isolated isolates of Mycobacterium tuberculosis in Beijing and other regions have a relatively close genetic relationship and a strong correlation at the genetic level. Mycobacterium tuberculosis may be transmitted more frequently in the male population than in women