目的　人子宫内膜分离培养并得到纯度较高的内膜基质细胞和腺体细胞。　方法　采用二次滤网过滤法进行分离并通过光镜观察和免疫细胞化学染色对其进行鉴别。　结果　基质细胞在接种后 0 5h开始贴壁 ,可见梭形和多角形两种形态的细胞。免疫细胞化学染色显示这两种细胞均具有波形蛋白免疫反应性 ,反应率可达95 %以上 ,而细胞角蛋白无免疫反应性 ,提示它们为来源于内膜基质的基质细胞。大部分腺体细胞在接种 2 4h后贴壁 ,培养后 4d左右腺体细胞呈旋涡状排列 ,单个细胞为多角形 ,核大而圆。腺细胞呈细胞角蛋白免疫反应性 ,反应率在 90 %以上。　结论　采用二次滤网过滤法可成功地分离人子宫内膜基质细胞和腺体细胞 The purpose of human endometrial separation culture and get higher purity of endometrial stromal cells and glandular cells. Methods The secondary filter was used for separation and identification by light microscopy and immunocytochemical staining. Results Stromal cells began to attach at 0 5 h after inoculation, showing two types of cells, spindle and polygon. Immunocytochemical staining showed that both of these two cells had vimentin immunoreactivity, with a response rate of over 95%, whereas cytokeratin was not immunoreactive, suggesting that they are stromal cells derived from the intima stroma. Most of the glandular cells adhered 24 hours after inoculation, and the glandular cells were arranged in a spiral shape after 4 days of culture. The single cells were polygonal with large and round nuclei. Gland cells cytokeratin immunoreactivity, the response rate of 90%. Conclusion The secondary filter can successfully separate human endometrial stromal cells and glandular cells