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目的:分析希佩尔-林道病(VHL)蛋白和血管内皮生长因子(VEGF)在家族性腺瘤性息肉病中的表达,并探讨其在腺瘤形成以及癌变过程中的可能作用机制。方法:收集我科2003年3月到2007年8月10例家族性腺瘤性息肉患者手术切除标本,分别取正常肠黏膜组织、≥1cm的腺瘤性息肉以及癌变腺瘤组织,采用SP法免疫组化方法检测VHL和VEGF在这些标本组织中的表达。结果:VHL阳性信号呈棕黄色主要表达于胞质内,VEGF阳性表达为棕黄色,主要表达于肿瘤细胞胞质。VHL在正常肠黏膜组织和≥1cm的腺瘤性息肉中的表达均较强,两者相差并不显著(χ2=2.4,P>0.05),但明显高于VHL基因在癌变腺瘤中的表达,差异具有统计学意义(χ2=7.2,P<0.05;χ2=14.4,P<0.05);VEGF在正常肠黏膜细胞、≥1cm的腺瘤性息肉到癌变腺瘤组织中阳性表达率不断升高,差异具有统计学意义(χ2=5.051,-P<0.05;χ2=11.52,P<0.05)。结论:VHL可能作为肿瘤抑制基因参与了家族性腺瘤性息肉的发展,VHL和VEGF血管内皮生长因子的异常表达与腺瘤到癌变的过程密切相关。
OBJECTIVE: To analyze the expression of Hippel-Lindau disease (VHL) protein and vascular endothelial growth factor (VEGF) in familial adenomatous polyposis and to explore its possible mechanism in adenoma formation and carcinogenesis. Methods: Surgical specimens of 10 patients with familial adenomatous polyps were collected from March 2003 to August 2007 in our department. Normal intestinal mucosa, adenomatous polyps ≥1 cm and adenocarcinoma of the cancerous tissue were collected respectively and immunized by SP method Histochemistry was used to detect the expression of VHL and VEGF in these specimens. Results: The positive signal of VHL was mainly expressed in the cytoplasm in brown and the positive expression of VEGF was brown-yellow, which was mainly expressed in the cytoplasm of tumor cells. The expression of VHL in normal intestinal mucosa and adenoma ≥1cm was strong, but the difference was insignificant (χ2 = 2.4, P> 0.05), but significantly higher than that of VHL in cancerous adenoma , The difference was statistically significant (χ2 = 7.2, P <0.05; χ2 = 14.4, P <0.05). The positive rate of VEGF in normal intestinal mucosa cells,> 1cm adenomatous polyps to cancerous adenoma was continuously increased , The difference was statistically significant (χ2 = 5.051, -P <0.05; χ2 = 11.52, P <0.05). CONCLUSION: VHL may participate in the development of familial adenomatous polyps as a tumor suppressor gene. The abnormal expression of VHL and VEGF VEGF is closely related to the carcinogenesis of adenomas.