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目的探讨结核分枝杆菌(Mtb)感染对小鼠B淋巴细胞亚群分布的影响。方法用Mtb大理临床分离株感染BALB/c小鼠。取不同感染阶段的小鼠腹腔细胞、脾脏细胞和淋巴结细胞,采用FACSCalibor检测分析Mtb感染早期及晚期小鼠的B细胞亚群变化。结果与对照组比较,小鼠感染Mtb临床分离株早期及晚期脾脏B细胞(CD45R+)百分率均降低[(8.11±3.08)%,t=3.031,P=0.013;(8.35±2.61)%,t=2.251,P=0.048],淋巴结B细胞(CD19+)百分率均升高[(41.20±8.25)%,t=-3.22,P=0.009;(36.29±6.99)%,t=-2.935,P=0.005];B1细胞百分率在感染早、晚期均无明显改变,B1b细胞(B220IntIgD-CD5-CD11+)百分率在早、晚期均降低[(4.89±3.43)%,t=2.248,P=0.048;(12.07±5.52)%,t=2.338,P=0.041],B1a细胞(B220IntIgD-CD5+CD11+)百分率在感染早期升高(50.58±9.50)%,t=-2.259,P=0.047;FOB细胞(B220+CD23+CD21int)升高(28.35±5.56)%,t=-2.571,P=0.028;MZ B细胞(B220+CD23-CD21~(hi))降低(15.80±6.10)%,t=3.142,P=0.01。结论 Mtb感染可影响小鼠B细胞及其亚群的分布,使机体更有利于抗结核感染。
Objective To investigate the effect of Mtb infection on the distribution of B lymphocyte subsets in mice. Methods BALB / c mice were infected with Mtb Dali clinical isolates. The peritoneal cells, spleen cells and lymph node cells of mice in different stages of infection were collected and analyzed by FACSCalibor to detect the changes of B cell subsets in early and late stage of Mtb infection. Results Compared with the control group, the percentages of CD45R + cells in the early and late stage of the infected Mtb in mice were significantly lower than those in the control group [(8.11 ± 3.08)%, t = 3.031, P = 0.013, (8.35 ± 2.61)%, t = P <0.001). The percentage of lymphocyte B cells (CD19 +) in the lymph nodes was significantly higher than that in the control group (2.251, P = 0.048) ; The percentage of B1 cells in the early and late stages of infection did not change significantly, and the percentage of B1b cells (B220IntIgD-CD5-CD11 +) decreased in the early and late stages (4.89 ± 3.43%, t = 2.248, P = 0.048; The percentage of B220IntIgD-CD5 + CD11 + in B1a cells increased 50.58 ± 9.50% at the early stage of infection, t = -2.259, P = 0.047. The percentages of B220 + CD23 + CD21int) was increased (28.35 ± 5.56)%, t = -2.571, P = 0.028; The MZ B cells decreased by (15.80 ± 6.10)%, t = 3.142, P = 0.01. Conclusion Mtb infection can affect the distribution of B cells and their subpopulations in mice, making the body more conducive to anti-TB infection.