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目的 :探讨Hedgehog(Hh)信号通路阻滞剂HPI-4对人上皮性卵巢癌A2780细胞增殖、周期分布和凋亡的影响。方法 :25、50、100和200μmol/L HPI-4处理人上皮性卵巢癌A2780细胞24、48和72 h后,应用MTT法检测细胞的增殖抑制率。25、50、100和200μmol/L HPI-4处理人上皮性卵巢癌A2780细胞48 h后,应用FCM法检测细胞周期及细胞凋亡情况,蛋白质印迹法检测细胞中cyclin D1和Gli1蛋白的表达。结果 :25、50和100和200μmol/L HPI-4处理24、48和72 h后,A2780细胞的增殖受到抑制,呈时间和浓度依赖性(P值均<0.05)。25、50、100和200μmol/L HPI-4处理48 h后,A2780细胞被阻滞于G0/G1期,细胞凋亡率上升,均呈浓度依赖性(P值均<0.05);A2780细胞中cyclin D1和Gli1蛋白的表达水平下调,呈浓度依赖性(P值均<0.05)。结论 :HPI-4能明显抑制人上皮性卵巢癌A2780细胞的增殖并能诱导其凋亡,且cyclin D1和Gli1蛋白的表达水平下调。
Objective: To investigate the effect of Hedgehog (Hh) signaling pathway inhibitor HPI-4 on proliferation, cell cycle distribution and apoptosis of human epithelial ovarian cancer A2780 cells. Methods: After treated with 25, 50, 100 and 200 μmol / L HPI-4 for 24, 48 and 72 h, the inhibitory rate of proliferation of human epithelial ovarian cancer cells A2780 was detected by MTT assay. After being treated with 25, 50, 100 and 200 μmol / L HPI-4 for 48 h, the cell cycle and apoptosis were detected by FCM. The expressions of cyclin D1 and Gli1 protein were detected by Western blotting. Results: Proliferation of A2780 cells was inhibited in 25, 40 and 72 h after being treated with 25, 50 and 100 μmol / L HPD-4 and HPI-4, both in a time and concentration-dependent manner (P <0.05). A2780 cells were arrested in G0 / G1 phase at 25, 50, 100 and 200 μmol / L HPI-4 for 48 h, the apoptosis rate increased in a concentration-dependent manner (P <0.05) The expression of cyclin D1 and Gli1 protein were down-regulated in a concentration-dependent manner (P <0.05). CONCLUSION: HPI-4 can significantly inhibit the proliferation and induce the apoptosis of human epithelial ovarian cancer A2780 cells, and the expression of cyclin D1 and Gli1 protein is down-regulated.