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建立益母草制剂中盐酸水苏碱与盐酸益母草碱的含量测定方法。采用LC-MS同时测定盐酸水苏碱与盐酸益母草碱的含量。使用Waters XBridge Amide色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%甲酸为流动相,流速1.0 m L·min-1,分流比为1∶4。质谱条件为ESI离子源,正离子模式,选择性离子扫描(SIM)下对盐酸水苏碱(m/z 144.0)和盐酸益母草碱(m/z312.0)进行测定。盐酸水苏碱浓度在0.562 8~281.4μg·L-1线性关系良好(r=0.999 8)。盐酸益母草碱浓度在0.521 2~260.6μg·L-1性关系良好(r=0.999 8)。该方法准确、简便、可靠,可作为益母草制剂的含量测定方法。
To establish a method for the determination of Leonurine hydrochloride and Leonurine hydrochloride in Motherwort preparation. Simultaneous Determination of Stachydrine Hydrochloride and Leonurine Hydrochloride by LC-MS. A Waters XBridge Amide column (4.6 mm × 250 mm, 5 μm) was used with mobile phase of acetonitrile-0.1% formic acid at a flow rate of 1.0 mL · min-1 with a split ratio of 1: 4. Mass spectrometry conditions were ESI ion source, positive ion mode, selective ion scan (SIM) stachydrine (m / z 144.0) and Leonurine (m / z 312.0) were determined. Stachydrine hydrochloride concentration in the 0.562 8 ~ 281.4μg · L-1 good linear relationship (r = 0.999 8). Concentration of Leonurine hydrochloride in the 0.521 2 ~ 260.6μg · L-1 sexual relationship was good (r = 0.999 8). The method is accurate, simple and reliable, and can be used as a method for the determination of Motherwort preparations.