论文部分内容阅读
目的:通过对不同三七选育品系的遗传变异和遗传分化程度进行分析比较,为三七的品种选育提供理论依据。方法:利用自行设计和他人开发的17对EST-SSR引物,对来自4个不同区域的17份三七选育品系进行遗传多样性及遗传分化分析。结果:在17份三七选育品系中一共扩增出136个多态位点,平均多态信息量PIC值为0.78,Nei′s基因多样性H0.139,Shannon多样性指数I0.208。选育品系间的遗传分化系数为0.382,遗传相似度和聚类分析的结果表明17份三七选育品系和屏边三七被划分为4个大类群,其中17份三七选育品系被分为3个类群,屏边三七单独在一个类群。结论:通过集团选择后,从相同栽培居群内筛选出的不同品系存在一定程度的遗传分化,可以用EST-SSR标记来检测集团选择的结果。
OBJECTIVE: To provide a theoretical basis for the breeding of Panax notoginseng through the analysis and comparison of the genetic variation and the degree of genetic differentiation of the different Panax notoginseng strains. Methods: 17 pairs of EST-SSR primers designed and developed by others were used to analyze the genetic diversity and genetic differentiation of 17 Panax notoginseng strains from 4 different regions. Results: A total of 136 polymorphic loci were amplified from 17 Panax notoginseng strains. The average PIC value was 0.78, Nei’s gene diversity was 0.139, and Shannon’s diversity index was 0.208. The genetic differentiation coefficient of the breeding lines was 0.382. The results of genetic similarity and cluster analysis indicated that 17 lines of Panax notoginseng and Panax notoginseng were divided into 4 groups, of which 17 were selected Divided into three groups, Panax notoginseng alone in a taxon. CONCLUSION: Through the selection of groups, different strains selected from the same cultivated population have a certain degree of genetic differentiation. EST-SSR markers can be used to detect the results of group selection.