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目的:观察槐定碱(sophoridine)致痫大鼠清醒核团脑电(IEEG)变化特点;研究痫样特征分类和致痫作用机制。方法:采用慢性埋藏电极记录清醒大鼠核团脑电(intracranial electroencepholography,IEEG)的方法,观察槐定碱对大鼠产生痫样放电的特点,并判断致痫原发部位;采用小鼠模型实施工具药拮抗实验,探讨槐定碱所致癫痫样发作的作用机制及类型。结果:海马齿状回(DG)内颗粒细胞对于槐定碱致痫作用最敏感,其次是内侧穿行通路(PP)和颞叶皮层(TC)。阈下催眠剂量的地西泮、催眠剂量的巴比妥钠可以对抗槐定碱引起的痫样惊厥的发生;对抗最大电休克剂量的苯妥英钠不能对抗槐定碱引起的痫样惊厥的发生,但可以减缓惊厥的发生时间和小鼠的死亡时间。结论:海马内部异常放电在槐定碱致痫作用中可能起到重要作用,海马部位可能是痫样发作的原发部位;槐定碱的致动物癫痫样发作属于临床小发作类型,地西泮是较理想的预防药物。
OBJECTIVE: To observe the characteristics of the changes of the electroencephalogram (IEEG) of soberhorn nuclei induced by sophoridine in rats and to study the classification of epileptiform features and the mechanism of epilepsy. Methods: Chronic burial electrodes were used to record the intracranial electroencephorography (IEEG) in rats. The sophoridine-induced discharge of epileptiform was observed in the rats and the primary site of epileptogenesis was determined. The mouse models were used Antagonistic experiment with drug to explore the mechanism and type of epileptiform attack caused by sophoridine. RESULTS: Granulosa cells in hippocampus dentate gyrus (DG) were most sensitive to epileptic sepsis, followed by medial pass (PP) and temporal cortex (TC). Subthreshold hypnosis dose of diazepam, hypnotic dose of sodium pentobarbital can be opposed to sophoridine-induced epileptic convulsions occurred; against the maximum electrical shock dose of phenytoin sodium can not be opposed to sophoridine-induced epileptic convulsions, But can reduce the time of onset of convulsions and the time of death in mice. Conclusion: The abnormal discharge in the hippocampus may play an important role in the epileptic effect of sophoridine. The hippocampus may be the primary site of epileptiform attack. The epileptiform epilepticus of sophoridine belongs to the type of clinical seizure. Diazepam Is the ideal preventive medicine.