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本文利用酸性聚丙烯酰胺凝胶电泳 ( A-PAGE,p H3 .1 )方法 ,研究了两个小麦回交 F2 群体 1 8个醇溶蛋白组分的遗传模式 ,根据醇溶蛋白带的出现与不出现特点 ,可以得到回交 F2 代各个组分的分离比例 ,然后利用 X2 分析检验其对不同基因假设模式的适合性 ,结果表明 ,1 5个醇溶蛋白带 ( α77,α79,α80 ,α84,α86,α88,α90 ,α92 ,β74,r54,r58,r59,w2 4 ,w2 6和 w34)受一个显性基因支配 ,两个带 ( r61 和 w33)分别受控于两个独立基因 ,其余 1条带由于在电泳谱上常表现模糊 ,其观察值 (出现∶不出现 )远低于期望值 ,因而它的遗传模式未能确定。另外还发现 4组蛋白带 ( α77-α80 ,α84-α86,α88-α92 和 r58-w2 4 -w2 6)总是一起遗传 ,暗示控制每组蛋白带的基因是紧密连锁的
In this paper, the genetic patterns of 18 prolamin components in F2 populations of two wheat cultivars were studied by acidic polyacrylamide gel electrophoresis (A-PAGE, p H3 .1). According to the appearance of prolamin bands The results showed that 15 gliadin bands (α 77, α 79, α 80, α 84 , α86, α88, α90, α92, β74, r54, r58, r59, w2 4, w2 6 and w34) are dominated by a dominant gene and the two bands (r61 and w33) are controlled by two independent genes, respectively 1 band due to the fuzzy performance of the electrophoretic spectrum, the observation (appearance: does not appear) is far below the expected value, so its genetic model can not be determined. It was also found that four sets of protein bands (α77-α80, α84-α86, α88-α92 and r58-w2 4 -w2 6) were always inherited together, suggesting that the genes controlling each protein band are tightly linked