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[目的]探讨引起食物中毒的致病性大肠埃希菌(EPEC)O111:K58的分离鉴定及血清学试验。[方法]依据GB/T 4789.6-2003对4份食物中毒患者肛拭或粪便进行培养分离,经染色镜检,生化反应,血清学试验作出鉴定,并对该起食物中毒进行流行病学调查。[结果]从食物中毒患者的3份肛拭和1份粪便均检出并确证为致病性大肠杆菌EPEC(O111:K58);并确定导致食物中毒的病因是由致病性大肠肝菌EPEC(O111:K58)引起。[结论]致病性大肠埃希菌引起的食物中毒可达到快速检验和鉴定。
[Objective] To investigate the isolation, identification and serological test of pathogenic E. coli O111: K58 causing food poisoning. [Method] According to GB / T 4789.6-2003, four swine swabs or feces were isolated and cultured in food poisoning patients. The samples were identified by stained microscopy, biochemical reactions and serological tests, and epidemiological investigation of the food poisoning was conducted. [Results] The three samples of anal swabs and one stool from food poisoning patients were both confirmed and confirmed as pathogenic E. coli EPEC (O111: K58). The cause of food poisoning was confirmed by pathogenic E. coli EPEC (O111: K58). [Conclusion] The food poisoning caused by pathogenic Escherichia coli can reach the rapid test and identification.