苹果褪绿叶斑病毒CLSV是影响苹果生产的主要潜隐病毒之一。目前在培育繁殖苹果无病毒苗木的工作中,普遍采用木本指示植物二重芽接法检测病毒,但这种方法费工费时。因此,我们从1987年开始进行病毒的分离提纯和抗血清制备工作,旨在为苹果潜隐病毒的大规模检测,建立简便、快速、灵敏的血清学方法。 Apple chlorotic leaf spot virus CLSV is one of the major latent viruses affecting apple production. At present, in breeding and breeding apple virus-free seedlings in the work, the common use of wood-based plants double bud method to detect the virus, but this method takes time and laborious. Therefore, we started in 1987 to isolate and purify viruses and prepare antiserums, aiming to establish a simple, rapid and sensitive serological method for large-scale detection of apple latent virus.