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目的探讨单一剂量的美法仑治愈荷瘤野生型C57BL/6小鼠的过程与肿瘤坏死因子α(TNFα)的关系。方法以3种遗传背景相同、肿瘤坏死因子受体1(TNFR1)基因型不同的TNFR1+/+、TNFR1+/-和TNFR1-/-C57BL/6小鼠为实验动物,皮下接种数量相同的小鼠淋巴瘤EL4细胞。接种瘤细胞后12d,给基因型不同的各组荷瘤小鼠腹腔内单次注射7.5mg/kg的美法仑。以荷瘤野生型C57BL/6小鼠(TNFR1+/+)为对照,观察美法仑对荷瘤TNFR1+/-C57BL/6小鼠和荷瘤TNFR1-/-C57BL/6小鼠的治疗效应。结果在美法仑(7.5mg/kg)治疗后的1周内,基因型不同的各组荷瘤小鼠肿瘤消退的速度基本相同。在随后的2月内,荷瘤TNFR1+/+和TNFR1+/-C57BL/6小鼠的肿瘤结节逐渐消退、肿瘤治愈;而多数荷瘤TNFR1-/-C57BL/6小鼠的肿瘤结节缩小后又再次出现并逐渐长大、肿瘤复发。结论TNFα与美法仑治愈肿瘤的过程密切相关,其中美法仑的抗肿瘤作用与荷瘤小鼠TNFR1的表达无关,但在美法仑治疗后,机体预防或避免肿瘤复发方面需要TNFR1在机体细胞的表达,而不是在肿瘤细胞的表达。
Objective To investigate the effect of single dose melphalan in curing tumor-bearing wild-type C57BL / 6 mice and its relationship with tumor necrosis factor-α (TNFα). Methods Three experimental mice with the same genetic background and different TNFR1 genotypes of TNFR1 + / +, TNFR1 +/- and TNFR1 - / - C57BL / 6 mice were inoculated subcutaneously with the same number of mouse lymph nodes Tumor EL4 cells. Twelve days after the inoculation of tumor cells, a single injection of 7.5 mg / kg melphalan was given intraperitoneally to each group of tumor-bearing mice of different genotypes. The therapeutic effects of melphalan on tumor-bearing TNFR1 +/- C57BL / 6 mice and tumor-bearing TNFR1 - / - C57BL / 6 mice were observed using tumor-bearing wild-type C57BL / 6 mice (TNFR1 + / +) as controls. Results In the 1 week after melphalan (7.5 mg / kg) treatment, tumors of tumor-bearing mice of different genotypes subsided at the same rate. In the following 2 months, tumor nodules of tumor-bearing TNFR1 + / + and TNFR1 +/- C57BL / 6 mice subsided gradually, and the tumors were cured; however, the tumor nodules of most tumor-bearing TNFR1 - / - C57BL / 6 mice were reduced Appears again and gradually grow up, tumor recurrence. Conclusion TNFα and melphalan are closely related to the tumor healing process. The anti-tumor effects of melphalan are not related to the expression of TNFR1 in tumor-bearing mice. However, after melphalan treatment, the body needs TNFR1 in the body to prevent or avoid tumor recurrence The expression of cells, but not in the tumor cells.