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目的探讨偏振数码照相结合计算机辅助图像分析法评价邻面去釉后釉质脱矿程度的准确性。方法于2010年3月选取青岛大学医学院附属医院口腔外科门诊因正畸需要拔除的上颌第一前磨牙40颗,沿牙冠颊舌向磨切得到80个标本,随机分为A(空白对照,未进行任何去釉处理)、B(邻面去釉)、C(邻面去釉后使用氟化泡沫)、D(邻面去釉后使用护牙素)4组。体外pH循环60d后,用偏振数码照相结合计算机辅助图像分析法定量测量各组标本邻面去釉及再矿化后的灰度值,以此来评价其釉质脱矿程度。同时用显微硬度仪定量测量各组标本的硬度值作为对照。结果 (1)灰度值:A组灰度值最低,B组灰度值最高,C组和D组经过再矿化处理后灰度值较B组降低,差异有统计学意义(P<0.05)。C组和A、D组之间灰度值差异无统计学意义(P>0.05)。(2)硬度值:A组硬度值最高,B组硬度值最低,C组和D组经过再矿化处理后硬度值升高,但均较A组低。B组硬度值和其他3组之间比较,差异均有统计学意义(P<0.05),而A组与C组、C组与D组之间硬度值比较,差异均无统计学意义(P>0.05)。结论偏振数码照相结合计算机辅助图像分析法得出的结果与显微硬度仪的定量检测结果相符合,表明了其是一种有效的定量评价釉质脱矿及再矿化效果的方法,值得临床推广。
Objective To discuss the accuracy of evaluating the degree of enamel demineralization by using the combination of polarized digital photography and computer-assisted image analysis. Methods In March 2010, 40 maxillary first premolar patients who had to be removed due to orthodontics in the oral surgery clinic of Affiliated Hospital of Medical College of Qingdao University were selected and 80 specimens were ground and ground along the buccal and lingual surfaces of the crown. They were randomly divided into A (blank control , Without any enamel treatment), B (adjacent to enamel), C (fluoridated foam after enamel use), D (enamel use with enamel). After 60 days of in vitro pH cycling, the degree of enamel demineralization was evaluated by polarization digital camera combined with computer-aided image analysis to quantitatively measure the gray values of enamel and remineralization of the adjacent samples. At the same time, the hardness of each group of specimens was measured quantitatively by using microhardness tester as control. Results (1) Gray value: the gray value of group A was the lowest, the gray value of group B was the highest. The gray value of group C and group D after remineralization was lower than that of group B, the difference was statistically significant (P <0.05 ). There was no significant difference in gray value between groups C and A, D (P> 0.05). (2) Hardness value: The hardness value of group A is the highest, the hardness value of group B is the lowest, the hardness values of group C and group D are increased after remineralization, but both are lower than group A. There were significant differences between the hardness values of group B and the other three groups (P <0.05), but there was no significant difference between group A and group C, group C and group D (P > 0.05). Conclusion The combination of polarization digital photography and computer-aided image analysis is in good agreement with the quantitative detection of microhardness tester, which shows that it is an effective method for quantitative evaluation of enamel demineralization and remineralization and is worthy of clinical promotion .