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目的建立同时测定人参药材主根、侧根、须根、芦头中13种人参皂苷类成分含量的高效液相色谱-蒸发光散射检测器(high performance liquid chromatography-evaporative light scattering detector,HPLC-ELSD)法。方法采用Venusil XBP C_(18)(L)色谱柱(250 mm×4.6 mm,5μm),流动相:乙腈-水梯度洗脱,流速:1 mL·min~(-1),柱温:20℃,蒸发光散射检测器参数:载气流量为2.9 L·min~(-1),压力为5.5×10~5Pa,漂移管温度为107℃,增益为1。结果人参皂苷Rg_1、Re、Rf、Rh_1、Rg_2、Rb_1、Rc、Rb_2、Rb_3、Rd、F_2、Rh_4、Rg_3分别在0.90~18.04、0.93~18.50、0.77~15.40、0.30~6.02、0.30~6.05、1.80~36.00、1.31~26.26、1.37~27.40、0.30~6.05、0.52~10.33、0.30~6.12、0.30~6.02、0.30~6.02μg内呈良好的线性关系(r>0.999 0),平均加样回收率(n=6)为97.1%~101.8%,RSD≤3.0%。结论该方法可用于同时测定13种人参皂苷的含量。
OBJECTIVE To establish a high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method for simultaneous determination of 13 ginsenosides in the main root, lateral root, fibrous root and root of Radix Ginseng. Methods A venusil XBP C_ (18) (L) column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was eluted with acetonitrile-water. The flow rate was 1 mL · min -1. The parameters of Evaporative Light Scattering Detector are as follows: the carrier gas flow rate is 2.9 L · min -1, the pressure is 5.5 × 10 ~ 5Pa, the drift tube temperature is 107 ℃ and the gain is 1. Results Ginsenosides Rg_1, Re, Rf, Rh_1, Rg_2, Rb_1, Rc, Rb_2, Rb_3, Rd, F_2, Rh_4 and Rg_3 were 0.90 ~ 18.04,0.93 ~ 18.50,0.77 ~ 15.40,0.30 ~ 6.02,0.30 ~ 6.05, 1.80 ~ 36.00,1.31 ~ 26.26,1.37 ~ 27.40,0.30 ~ 6.05,0.52 ~ 10.33,0.30 ~ 6.12,0.30 ~ 6.02,0.30 ~ 6.02μg within a good linear relationship (r> 0.999 0), the average recovery rate (n = 6) was 97.1% ~ 101.8%, RSD≤3.0%. Conclusion This method can be used to determine the content of 13 kinds of ginsenosides simultaneously.