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晋谷21号幼穗从诱导愈伤组织到分化成苗需要两步完成，再生率达60％－80％。诱导愈伤组织培养基A1和A8较好，分化培养基B1与B2较好。A1上的愈伤组织在B2上的分化率比B1高15％，A3上的愈伤组织在B1上的分化率比B2约高20％。长根培养基用C2优于C1，C2上的苗移栽成活率比C1高10％－20％。愈伤组织随着继代次数增加，分化再生率逐渐降低。
Jinya 21 young panicle from inducing callus to differentiate into two steps need to complete the regeneration rate of 60% -80%. Callus induction medium A1 and A8 were better, and differentiation medium B1 and B2 were better. The differentiation rate of callus on A1 was 15% higher than that of B1 and the differentiation rate of callus on A3 was about 20% higher than that of B2. C2 is better than C1 in rooting medium, and the transplanting survival rate on C2 is 10% -20% higher than C1. As the number of subcultures increased, the rate of differentiation and regeneration gradually decreased.