论文部分内容阅读
目的筛选食管癌细胞增殖培养液中大鼠血清的最佳浓度和活性状态。方法采用常规饲料喂饲大鼠30d,股动脉采血制备血清,采用不同浓度和活性状态的大鼠血清培养液培养人食管上皮鳞癌细胞株Eca-109,采用四甲基偶氮噻唑蓝(MTT)法,筛选大鼠血清培养液对Eca-109细胞生长增殖的最佳作用条件,以小牛血清培养液及人正常肝上皮细胞株HL7702作为对照进行比较。结果各浓度未灭活大鼠血清培养的人食管癌细胞Eca-109基本上均呈指数增长趋势,72h时各浓度组间差异有统计学意义(F=159.263,P<0.05);用5%未灭活大鼠血清培养人食管癌细胞Eca-109和人正常肝上皮细胞HL7702,培养72h时,2株细胞的生长增殖能力均强于5%灭活大鼠血清,差异均有统计学意义(F=37.746,P<0.05;F=43.399,P<0.05);与小牛血清对2株细胞生长增殖的作用接近(P>0.05)。结论 5%相同浓度条件下,未灭活大鼠血清培养液更适合细胞生长。
Objective To screen the optimal concentration and activity of rat serum in esophageal cancer cell proliferation medium. Methods The rats were fed with conventional diet for 30 days and the blood was collected from the femoral artery to prepare the human esophageal epithelial squamous cell carcinoma cell line Eca-109. The rat esophageal squamous cell carcinoma cell line Eca-109 was cultured in different concentrations and concentrations. MTT ) Method was used to screen the optimal conditions for the growth and proliferation of Eca-109 cells in the rat serum culture medium. Bovine serum culture medium and human normal liver epithelial cell line HL7702 were used as controls. Results Eca-109 of human esophageal cancer cells cultured in serum of each group did not show significant exponential growth trend at all concentrations (all P <0.05) Eca-109 and human normal liver epithelial cells HL7702 were cultured in non-inactivated rat serum, and the proliferation of 2 cells was stronger than that of 5% inactivated rat serum at 72 hours after culture, the differences were statistically significant (F = 37.746, P <0.05; F = 43.399, P <0.05). The effect of bovine serum on proliferation and proliferation of the two cell lines was similar (P> 0.05). Conclusion Under the same concentration of 5%, the inactivated rat serum culture medium is more suitable for cell growth.