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目的:比较核酸富集处理前后3种核酸提取方法对精炼食用植物油的提取效果。方法:基于核酸具有水溶性的原理,将大量植物油样中的核酸最大可能的萃取富集到少量具有细胞裂解功能的水相中,再以此水溶性液体为对象,采用两种试剂盒法和经典的CTAB核酸抽提方法进行后续提取步骤,以植物叶绿体tRNA基因片段的扩增结果作为各种方法核酸提取效果的评估指标。结果:核酸富集处理前,采用3种核酸提取方法在其核酸提取液中都未扩增出叶绿体tR-NA基因片段。核酸富集处理后,在12个精炼植物油中,采用DNeasy Plant Mini Kit试剂盒法,只有3个样品的核酸提取液未扩增出目的片段;采用Food EX Magnetic试剂盒法,有6个样品的核酸提取液扩增出目的片段;采用CTAB法,只有2个样品的核酸提取液未扩增出目的片段。结论:采用核酸富集处理后,3种提取方法的核酸提取效率都显著提高。
OBJECTIVE: To compare the effects of three nucleic acid extraction methods on the edible vegetable oil extraction before and after nucleic acid enrichment. Methods: Based on the principle of water-soluble nucleic acid, the nucleic acids in a large amount of vegetable oil samples were enriched to a small amount of the aqueous phase with cell lysis function, and then the water-soluble liquid was used as the object. The classical CTAB nucleic acid extraction method for subsequent extraction steps to plant chloroplast tRNA gene fragment amplification results as a method of evaluating the effect of nucleic acid extraction. Results: Before nucleic acid enrichment, the tR-NA gene fragment of chloroplast was not amplified by three kinds of nucleic acid extraction methods in its nucleic acid extract. After the nucleic acid enrichment, only 12 samples of refined vegetable oil were extracted with the DNeasy Plant Mini Kit, only 3 samples of the nucleic acid extract did not amplify the target fragment. Using the Food EX Magnetic kit, there were 6 samples Nucleic acid extract amplified the target fragment; using CTAB method, only two samples of the nucleic acid extract did not amplify the target fragment. Conclusion: The nucleic acid extraction efficiency of three kinds of extraction methods were significantly increased after nucleic acid enrichment.