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目的:建立HPLC法测定穿心莲内酯树脂复合物中穿心莲内酯含量的方法。方法:Hypersil C18柱,以甲醇-水(55∶45)为流动相,检测波长225 nm。结果:穿心莲内酯进样量在(0.095 6~0.956)μg范围内线性关系良好,回归方程为Y=230.4X+47.264,r=0.999 4(n=6),平均加样回收率为99.99%(n=9),RSD为0.88%。结论:该方法准确、可靠、重复性好,可作为穿心莲内酯树脂复合物中穿心莲内酯的含量测定方法。
Objective: To establish a HPLC method for the determination of andrographolide in andrographolide resin complex. Method: Hypersil C18 column with methanol-water (55:45) as mobile phase and detection wavelength at 225 nm. RESULTS: The linear relationship was well within the range of (0.095 6-0.956) μg for andrographolide injection. The regression equation was Y=230.4X+47.264, r=0.999 4 (n=6), and the average recovery was 99.99%. (n=9), RSD was 0.88%. Conclusion: The method is accurate, reliable and reproducible. It can be used as a method for determination of andrographolide in andrographolide resin complex.