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目的:建立UPLC-PDA法同时测定三黄方药液中黄柏碱、黄芩苷、小檗碱、黄芩素、芦荟大黄素、汉黄芩素、大黄酸、大黄素、大黄酚、大黄素甲醚10种成分的含量。方法:采用Waters ACQUITY BEH C18(2.1 mm×100 mm,1.7μm)色谱柱,以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,流速0.3 m L·min~(-1),柱温30℃,检测波长284 nm。结果:在一定浓度范围内,三黄方药液中10种成分均呈良好的线性关系(r>0.999);平均加样回收率为96.73%~101.7%(RSD<3%,n=6)。3批样品中黄柏碱、黄芩苷、小檗碱、黄芩素、芦荟大黄素、汉黄芩素、大黄酸、大黄素、大黄酚、大黄素甲醚含量分别在0.226~0.233、5.85~6.00、1.92~2.00、0.140~0.151、0.031 4~0.037 5、0.043 6~0.044 7、0.081 5~0.083 1、0.032 8~0.040 6、0.102~0.105、0.340~0.350 mg·mL~(-1)。结论:经方法学验证,本法可用于三黄方药液的质量控制。
Objective: To establish a UPLC-PDA method for the simultaneous determination of cortex phellodendri, baicalin, berberine, baicalein, aloe emodin, wogonin, rhein, emodin, chrysophanol, The composition of the content. Methods: The mobile phase was eluted with a gradient of acetonitrile (0.1% phosphoric acid) with a mobile phase of 0.3 mL · min -1 using a Waters ACQUITY BEH C18 column (2.1 mm × 100 mm, 1.7 μm) ℃, detection wavelength of 284 nm. Results: Within a certain concentration range, the 10 components in Sanhuang Fang liquid showed a good linear relationship (r> 0.999). The average recoveries were 96.73% ~ 101.7% (RSD <3%, n = 6) . The contents of cypermethrin, baicalin, berberine, baicalein, aloe-emodin, wogonin, rhein, emodin, chrysophanol and physcion in the three batches of samples were 0.226 ~ 0.233, 5.85 ~ 6.00 and 1.92 ~ 2.00,0.140 ~ 0.151,0.031 4 ~ 0.037 5,0.043 6 ~ 0.044 7,0.081 5 ~ 0.083 1,0.032 8 ~ 0.040 6,0.102 ~ 0.105,0.340 ~ 0.350 mg · mL -1. Conclusion: This method can be used for the quality control of Sanhuang Fang liquid by methodological verification.