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为了研究肺血管内皮细胞的异质性以及信息传递及其对血管运动的调控作用,本实验建立了家兔肺微动脉内皮细胞的非酶性分离、收集方法并进行原代及传代培养,观察了培养过程的细胞生长特性。肺微动脉内皮细胞的非酶性采集经微载体法进行。首先,处死动物后开胸并迅速行肺动脉及左心房插管术。
In order to study the heterogeneity of pulmonary vascular endothelial cells as well as the information transmission and the regulation of vascular movement, we established a non-enzymatic separation and collection method of rabbit pulmonary arteriolar endothelial cells and primary and subculture The cell growth characteristics of the culture process. Non-enzymatic capture of pulmonary arteriolar endothelial cells was performed by microcarrier method. First, the animals were sacrificed and then thoracotomy and pulmonary artery and left atrium cannulation were performed rapidly.