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1.以感染病毒后的鸡胚为抗元(冻融法)同恢复期血清试验中,血清阳性率21.4％,以正常鸡胚为抗元,所出现的血清非特异反应率24.7％,所谓抗元阳性率高达90％以上,由于非特异反应强和各正常鸡胚抗元性能上的差异,最后认为此种抗元在补体结合试验中,实用价值不大。2.消除非特异性反应:血清方面,认为六种处理方法中的吸收后再通以CO_2的方法较好,能消除半数血清非特异性反应,但要影响特异效价。抗元方面,加温处理无明显效果,经乙醚处理,可降低非特异性的强度,但敏感性要减低,同时特异性问题,仍未解决。3.用豚鼠肝脏抗元作补体结合反应;血清阳性率较高43.5％,非特异反应较低7.6％,且各正常豚鼠肝脏抗元性能上的差异不大。因此认为此种抗元比鸡胚抗元为佳。4.对豚鼠肝脏抗元,共作了16例的双分血清试验,其中有三例黄疸型肝炎的恢复期血清比早期血清增长4倍效价,但对正常抗元,有一例也出现了4倍增长。至于此种反应性质,文中作了简略讨论,并认为此种抗元具有一定特异性的效能。
1. The seroprevalence rate was 21.4% in serum samples after infection with chicken embryos (freeze-thaw method) and convalescence, and normal chicken embryos were used as the antivirals. The non-specific response rate of serum was 24.7%. The rate of resistance to positive cells was as high as 90%. Due to the strong non-specific response and the differences in the anti-cell properties of normal chicken embryos, this antibody was considered to be of practical value in the complement fixation test. 2. Elimination of non-specific reactions: In the serum, it is considered that the method of CO_2 after absorption in the six treatment methods is better, and half of the serum non-specific reactions can be eliminated, but the specific titer is to be affected. In terms of resistance, heat treatment has no obvious effect. Ether treatment can reduce the non-specific strength, but the sensitivity should be reduced. At the same time, the specificity problem has not yet been solved. 3. The guinea pig liver was used as the complement binding reaction; the seroprevalence was 43.5% higher, the non-specific response was lower 7.6%, and the difference in the liver resistance of the normal guinea pigs was not significant. Therefore, it is considered that this kind of resistance is better than chicken embryo resistance. 4. In the guinea pig liver resistance, a total of 16 double serum tests were performed. Three cases of convalescent jaundice had a 4 fold higher titer than those of early sera, but 4 of them had a normal resistance. Times increase. As for the nature of this reaction, a brief discussion was made in this article and it is considered that such anti-elements have certain specific effects.