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大田栽培条件下,研究了3种转植酸酶(phyA2)基因事件C63、C83-1-7和C84-1-14玉米根系分泌的植酸酶对土壤酸性磷酸酶活性、土壤磷素有效性以及作物磷积累量的影响。结果表明,3种转基因玉米根系植酸酶活性远远高于阴性对照,显著提高了根际土壤酸性磷酸酶的活性、增加了对根际土壤有机磷的活化和利用、减少了土壤速效磷的降低;与阴性对照相比,转phyA2基因玉米生长发育状况更好,C63和C84-1-14植株干重和磷积累量显著高于阴性对照,磷积累量分别是阴性对照的4.3倍和3.4倍。初步说明在土壤条件下,转phyA2基因玉米对土壤有机磷的利用增加,土壤磷素有效性显著提高。
Under field cultivation, the phytase excreted by maize roots of three phytase gene events C63, C83-1-7 and C84-1-14 were tested for their activities on soil acid phosphatase activity, soil phosphorus availability And the impact of crop phosphorus accumulation. The results showed that the phytase activities of three kinds of transgenic maize roots were much higher than those of the negative control, which significantly increased the activities of acid phosphatase in the rhizosphere soil, increased the activation and utilization of organic phosphorus in the rhizosphere soil and decreased the content of available phosphorus Compared with the negative control, the transgenic plants had better growth and development of maize, and the dry weight and phosphorus accumulation of C63 and C84-1-14 plants were significantly higher than those of the negative control plants. The phosphorus accumulation was 4.3 times and 3.4 times that of the negative control respectively Times Preliminary results showed that under soil conditions, the utilization of phyA2 gene maize increased soil organic phosphorus, soil phosphorus availability significantly increased.