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目的:为研究复合生物杀菌剂F6-11毒性,采用动物实验法进行毒理学评价。方法:对复合生物杀菌剂F6-11进行小鼠急性毒性试验、小鼠骨髓嗜多染红细胞微核试验、亚急性毒性试验、家兔多次完整皮肤刺激试验、急性眼刺激试验、豚鼠皮肤变态反应试验、鱼类延长毒性14天试验。结果:复合生物杀菌剂F6-11对小鼠急性毒性LD50>5000 mg/kg.bw,属于实际无毒级物质;小鼠微核试验该杀菌剂各剂量组与阴性对照组比较,微核率无显著性差异(P>0.05);亚急性毒性试验动物血常规、生化指标及各脏器均未发现异常;对家兔多次完整皮肤及眼刺激反应积分均为0,均属无刺激性;对豚鼠皮肤变态反应试验组动物与阴性对照组无可见不同,试验组动物皮肤致敏反应积分为0,无致敏作用;鱼类延长毒性14天试验无异常。结论:毒理学研究表明,复合生物杀菌剂F6-11具有良好的使用安全性。
Objective: To study the toxicity of compound biocide F6-11, toxicology was evaluated by animal experiment. Methods: Acute toxicity test of mice was carried out on the composite biocide F6-11. Micronucleus test of mouse bone marrow polychromatic erythrocytes, subacute toxicity test, multiple intact skin irritation test of rabbits, acute eye irritation test, guinea pig skin metamorphosis Response test, fish to extend toxicity 14 days test. Results: The compound biocide F6-11 had acute toxicity to mice with LD50> 5000 mg / kg.bw, which belonged to the actual non-toxic substances. Compared with the negative control group, the micronucleus rate No significant difference (P> 0.05); subacute toxicity test animals blood routine, biochemical indicators and all organs were found no abnormalities; repeated rabbit multiple intact skin and eye irritation response scores were 0, are non-irritating No significant difference was observed in the skin allergy test group of guinea pigs between the animals and the negative control group. The skin sensitization reaction score of the animals in the test group was 0 without any sensitization. No abnormalities were observed in the 14-day prolonged toxicity test of fish. Conclusion: Toxicological studies have shown that the composite biocide F6-11 has good safety.