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本文比较了不同酶液、渗透压稳定剂、酶解温室及菌丝培养基成份等因素对木耳属(Auricularia)中木耳(Auricularia auricula)和毛木耳(Auricularia polytricha)菌丝释放原生质体的作用及影响。用0.5%纤维素酶加0.5%蜗牛酶的混合酶液,以0.6M的MgSO_4为稳定剂,在34℃下可自两种菌丝体获得大量原生质体。对原生质体再生条件的研究表明,纤维二糖和菌丝体培养物浸提物对再生有明显促进作用,再生率达20%左右。本文还用VBL型荧光增白剂观察了菌丝脱壁以及原生质体细胞壁再生的过程。
In this paper, the effects of different enzyme solutions, osmotic stabilizers, enzymatic hydrolysis greenhouse and mycelial media on the release of protoplasts from Auricularia auricula and Auricularia polytricha were studied. influences. A large amount of protoplasts were obtained from both mycelia at 34 ℃ with 0.5% cellulase plus 0.5% snail enzyme mixed solution and 0.6M MgSO_4 as stabilizer. Studies on the regeneration conditions of protoplasts showed that cellobiose and mycelium extracts had a significant promotion on regeneration with a regeneration rate of about 20%. In this paper, VBL-type optical brightener was also used to observe the process of mycelial desolvation and protoplast cell wall regeneration.