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近年来,小西葫芦黄化花叶病毒(zucchini yellow mosaic virus,ZYMV)在黄瓜(Cucumis sativus L.)上危害愈发严重,影响黄瓜植株的生长发育,降低黄瓜的产量与品质。由于ZYMV缺乏有效的防治药剂,因此需要培育ZYMV抗性黄瓜品种。以ZYMV Z5-1为病毒源,对不同黄瓜材料的ZYMV抗性进行鉴定,并对抗性位点候选基因进行了测序分析。结果显示,黄瓜品种SA02、SA06、S94、S1003、WI7230与TMG-1具有ZYMV抗性,其抗性候选基因Csa6G152960.1的突变位点一致。在ZYMV抗性鉴定的基础上,通过回交转育,将黄瓜自交系TMG-1中的ZYMV抗性基因转入感病自交系SA0422中,回交期间利用分子标记对回交群体进行前景选择和背景选择,经过3代回交,将ZYMV抗性基因转入SA0422,为培育抗ZYMV黄瓜新品种奠定了基础。
In recent years, zucchini yellow mosaic virus (ZYMV) is more and more harmful to cucumber (Cucumis sativus L.), which affects the growth and development of cucumber plants and reduces the yield and quality of cucumber. Because ZYMV lacks an effective control agent, it is necessary to cultivate ZYMV-resistant cucumber varieties. ZYMV Z5-1 was used as the virus source to identify the ZYMV resistance of different cucumber materials. The candidate loci of the resistant loci were sequenced and analyzed. The results showed that the cucumber varieties SA02, SA06, S94, S1003, WI7230 and TMG-1 had ZYMV resistance, and the mutation site of the resistance candidate gene Csa6G152960.1 was consistent. Based on the identification of ZYMV resistance, the ZYMV resistance gene in cucumber inbred line TMG-1 was transferred into susceptible inbred line SA0422 by backcrossing and backcrossing. Prospective selection and background selection. After 3 generations of backcrossing, ZYMV resistance gene was transferred to SA0422, which lays a foundation for breeding new varieties of cucumber against ZYMV.