利用B超观察主动脉弓狭窄(transverse aortic constriction,TAC)小鼠心脏随着手术时间的延长所发生的动态变化,探索如何利用病理学方法判断心脏肥厚性改变。以微创手术小鼠TAC模型为研究对象,同步采集不同时间点动物心脏进行B超和组织切片观察,通过心脏B超找出针对该模型动物心脏肥厚的病理学诊断方法。结果显示:病理切片采集的室间隔、左心室厚度与B超采集的相应指标,不具有相关性;而室间隔/右室壁厚度比与B超采集的室间隔、左心室厚度具有明确的相关性,随着手术后时间的延长,心肌细胞也逐渐增大。结果表明:小鼠在接受微创主动脉狭窄手术后,单独B超检查就可以准确地发现心肌肥厚性的改变,而从病理组织学观察来看,单纯的左心室厚度不能作为心肌肥厚的标准,而通过室间隔/右室壁厚度比,以及心肌细胞的大小,可以更准确的反应心肌的肥厚性改变。 Using B ultrasound to observe the dynamic changes of heart in transverse aortic constriction (TAC) mice with the extension of operation time, and to explore how to use pathological methods to judge the changes of cardiac hypertrophy. The TAC model of minimally invasive surgery mice was used as the research object. The animal heart was collected at different time points for B-ultrasound and histological section observation. The pathological diagnosis of cardiac hypertrophy in this animal model was found by heart B ultrasound. The results showed that there was no correlation between the interventricular septum and the thickness of left ventricle collected by pathological section and the corresponding indexes of B-ultrasonography, while the interventricular septum / right ventricular wall thickness ratio had a clear correlation with the interventricular septum and left ventricular thickness With the extension of time after surgery, myocardial cells also gradually increased. The results showed that: after receiving minimally invasive aortic stenosis surgery in mice, B-ultrasound alone can accurately detect changes in cardiac hypertrophy, and histopathological observations, the simple left ventricular thickness can not be used as the standard of myocardial hypertrophy , And through the ventricular septum / right ventricular wall thickness ratio, as well as the size of myocardial cells, can more accurately reflect the hypertrophy of myocardium.