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目的考察不同提取方法对水蛭活性成分的提取效果,优化出水蛭活性成分配方,制备水蛭多肽气雾剂。方法以凝血酶时间为指标,采用脱脂、水提醇沉、胃蛋白酶酶解法分别提取水蛭活性成分,检测不同水蛭多肽溶液配方的稳定、防腐、防沉性,并自主设计微型超声雾化器。结果胃蛋白酶酶解法提取效果最佳,水蛭多肽溶液优选配方为水蛭胃蛋白酶酶解上清液∶甘油(V/V)1∶1.5,甘露醇2g/L,山梨酸钾1.5g/L。结论水蛭多肽气雾剂,鼻腔吸入,给药自行控制,使用方便,不需面罩,无浪费现象。
OBJECTIVE To investigate the effects of different extraction methods on the extraction of active ingredients of leeches, and to optimize the formulation of the active ingredients of leech, to prepare leech polypeptide aerosols. Methods Taking the time of thrombin as index, the active ingredients of leeches were extracted by degreasing, alcohol precipitation and pepsin respectively. The stability, anticorrosion and anti - sinking ability of different leeches polypeptide solutions were tested, and the miniature ultrasonic nebulizer was designed independently. Results Pepsin enzymatic extraction method was the best. The leech peptide solution preferred leech pepsin hydrolyzate supernatant: glycerol (V / V) 1: 1.5, mannitol 2g / L, potassium sorbate 1.5g / L. Conclusion Leech peptide aerosol, nasal inhalation, administration of self-control, easy to use, no mask, no waste phenomenon.