论文部分内容阅读
目的 观察分化的NT2 细胞大鼠脑内移植后的生存情况 ,以及bcl 2基因对移植神经元生存的影响 ;方法 用bcl 2转染NT2 细胞株 ,在培养基中加入 10 μm视黄酸进行分化诱导 5wk ,被诱导分化的细胞表现出成熟神经元的特性。对分化的细胞进行分离、纯化后移植到大鼠的海马。术后 16d及 6 0d分别处理大鼠 ,采用漂染法对大鼠脑组织切片进行免疫组织化学检测移植的神经元在大鼠海马区的生存情况 ;结果 有相当数量的NT2 细胞被视黄酸进行分化诱导成神经元 ,包括带有或不带有bcl 2基因的神经元均可在大鼠海马存活 2mo以上 ,带有bcl 2基因的神经元在大鼠海马存活的情况优于不带bcl 2基因的对照组 ,但在 6 0天时点带有或不带有bcl 2基因的神经元存活数量均低于第 16天时点 ;结论 由NT2 细胞株诱导分化的神经元大鼠海马移植后可生存相当一段时间 ,bcl 2基因有助于这种生存。移植的细胞数随时间延长而减少可能是由于大鼠免疫排斥形成所致
OBJECTIVE: To investigate the survival of rat NT2 cells after transplanted into the brain and the effect of bcl2 gene on the survival of transplanted neurons. Methods NT2 cells were transfected with bcl2 and differentiated into medium with 10 μm retinoic acid Induced 5wk, induced differentiation of the cells showed the characteristics of mature neurons. The differentiated cells were isolated, purified and transplanted into the rat hippocampus. The rats were treated on the 16th and 60th day after operation, and the survival of the transplanted neurons in the rat hippocampus was detected by immunohistochemical staining. The results showed that a considerable number of NT2 cells were treated with retinoic acid Differentiation-induced neurons, including neurons with or without bcl 2 gene, survived more than 2 months in rat hippocampus and neurons with bcl 2 gene survived in rat hippocampus better than those without bcl 2 However, the number of neurons with or without bcl 2 gene at 60 days was lower than that at day 16. Conclusion The hippocampus of rat induced by NT2 cell line can survive after transplantation For a long time, bcl 2 gene contribute to this kind of survival. Transplanted cells decreased with time may be due to the formation of immune rejection in rats