论文部分内容阅读
目的研究血管瘤组织中β肾上腺素能受体(β-AR)的密度和亲和力,并探讨其在血管瘤发病中的意义。方法采用放射标记的非选择性β-AR阻滞剂125I-吲哚洛尔、选择性β1-AR阻滞剂阿替洛尔,对12例增生期血管瘤组织和10例退化期血管瘤组织的β-AR进行双位点竞争结合实验,并根据双位点数学模型,测算出增生期血管瘤和退化期血管瘤组织中的β1-AR及β2-AR的密度和亲和力(抑制常数)。结果增生期血管瘤和退化期血管瘤组织中均存在高亲和力的β1-AR和低亲和力的β2-AR。增生期血管瘤组织中β1-AR的亲和力(0.26±0.09nmol/L)和β2-AR的亲和力(2.94±0.31nmol/L)分别与退化期相比(0.27±0.06nmol/L,2.91±0.53nmol/L),差异均无统计学意义(均P>0.05)。增生期血管瘤组织中β1-AR的密度(47.31±7.76 fmol/mg protein)和β2-AR的密度(172.21±30.56fmol/mg protein)分别高于退化期(24.09±5.17fmol/mg protein,73.48±13.34fmol/mg protein),且均有统计学意义(均P<0.01)。结论血管瘤组织中存在β1-AR和β2-AR,且以β2-AR为主;增生期血管瘤组织中β1-AR和β2-AR的密度明显上调而亲和力无明显变化;血管瘤的增生可能与其β1-AR和β2-AR尤其是β2-AR的密度上调有关。
Objective To study the density and affinity of β-adrenergic receptor (β-AR) in hemangiomas and to explore its significance in the pathogenesis of hemangiomas. Methods 125I-pindolol, a selectiveβ1-AR blocker, and radiolabeled non-selective beta-AR blocker were used in this study. Twelve cases of proliferative hemangiomas and 10 cases of degenerative hemangiomas Β-AR. The density and affinity (inhibition constants) of β1-AR and β2-AR in proliferating hemangiomas and degenerative hemangiomas were calculated according to the two-site mathematical model. Results Both high-affinity β1-AR and low-affinity β2-AR existed in both proliferative hemangioma and degenerative hemangioma. The affinity of β1-AR (0.26 ± 0.09nmol / L) and the affinity of β2-AR (2.94 ± 0.31nmol / L) in proliferating hemangiomas were significantly lower than that in the degenerative phase (0.27 ± 0.06nmol / L, 2.91 ± 0.53 nmol / L), the difference was not statistically significant (all P> 0.05). The density of β1-AR (47.31 ± 7.76 fmol / mg protein) and β2-AR (172.21 ± 30.56fmol / mg protein) in proliferating hemangiomas were higher than that in the degenerative stages (24.09 ± 5.17fmol / mg protein, 73.48 ± 13.34fmol / mg protein), all of which were statistically significant (all P <0.01). Conclusions β1-AR and β2-AR exist in the hemangiomas with predominant β2-AR. The density of β1-AR and β2-AR in the proliferative hemangiomas are significantly increased but the avidity is not significantly changed; the proliferation of hemangiomas may be Which is related to the up-regulation of β1-AR and β2-AR, especially β2-AR.