Anaerobic fermentation can increase biomass energy use efficiency of crop straws and realize win-win of energy and environment.This paper explored the biogas generation performance of anaerobic digestion of cow dung liquid as nitrogen source in three different levels of stirring intensity at 30℃ constant temperature condition. Through p H value,biogas production,chemical oxygen demand( COD),methane content,volatile fatty acid( VFA),principal component analysis( PCA) and modified Gompertz model,effects of agitating intensity on anaerobic digestion performance of corn straw silage were evaluated. Results indicate that the COD removal rate of three agitating intensity levels is higher than 85%,and p H value is about 6.5; the cumulative biogas production after 20 days is 2h > 4h > 1h of agitating; in the49 th day,the biogas production is 1.9 Lat 30 min /2h,1.7 L at 30 min /4 h,and 1. 6 Lat 30 min / h; the maximum biogas production rate is 30 min /2h > 30 min /4h > 30 min / h; and the maximum methane production rate is 30 min /4h > 30 min /2h > 30 min / h; in the same energy consumption,the biogas production at 30 min /4h is higher than 1h. In conclusion,overall analysis of energy consumption and economic factors indicate that 30 min /4 h agitating intensity is more suitable for straw biogas fermentation project. This study is expected to provide theoretical foundation for biogas fermentation project. Anaerobic fermentation can increase biomass energy use efficiency of crop straws and realize win-win of energy and environment. This paper explored the biogas generation performance of anaerobic digestion of cow dung liquid as nitrogen source in three different levels of stirring intensity at 30 ° C constant temperature condition. Through p H value, biogas production, chemical oxygen demand (COD), methane content, volatile fatty acid (VFA), principal component analysis (PCA) and modified Gompertz model, effects of agitating intensity on anaerobic digestion performance of corn straw silage Results expressed that COD removal rate of three agitating intensity levels is higher than 85% and p H value is about 6.5; the cumulative biogas production after 20 days is 2h> 4h> 1h of agitating; in the 49 th day, the biogas production is 1.9 Lat 30 min / 2h, 1.7 L at 30 min / 4 h, and 1.6 Lat 30 min / h; the maximum biogas production rate is 30 min / 2h> 30 min / 4h> 30 min / h ; and the max In conclusion, the overall analysis of energy consumption and economic factors was 30 min / 4h> 30 min / h; in the same energy consumption, the biogas production at 30 min / 4h is higher than 1h. indicate that 30 min / 4 h agitating intensity is more suitable for straw biogas fermentation project. This study is expected to provide theoretical foundation for biogas fermentation project.