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为增强DNA疫苗在体内诱导细胞毒性T细胞（CTL）的能力,作者构建了表达1型人类免疫缺陷症病毒（HIV-1）包膜和gag/pol蛋白的DNA疫苗（pCEnv、pCGag/pol）以及表达CD80和CD86的质粒（pCD80、pCD86）。对6～8周龄BALB/c小鼠的后肢肌肉以100μl 0.25％丁哌卡因盐酸预处理,2天后分3组进行免疫,剂量为:pCEnv+pCD80各50μg、pCEnv+pCD86各50μg以及pCEnv50μg（溶于PBS中）。两周后以铬释放法测CTL应答。发现pCEnv+pCD86可显著增强主要组织相容性Ⅰ类限制性及CD8~+T细胞依赖性CTL应答。
To enhance the ability of DNA vaccines to induce cytotoxic T cells (CTLs) in vivo, the authors constructed a DNA vaccine (pCEnv, pCGag / pol) expressing the envelope and gag / pol proteins of type 1 human immunodeficiency virus (HIV- As well as plasmids expressing CD80 and CD86 (pCD80, pCD86). Hindlimb muscles of 6-8 week old BALB / c mice were pretreated with 100 μl of 0.25% bupivacaine hydrochloride and immunized in 3 groups after 2 days in 50 μg of pCEnv + pCD80, 50 μg of pCEnv + pCD86 and 50 μg of pCEnv (Dissolved in PBS). Two weeks later, the CTL response was measured by chromium release assay. It was found that pCEnv + pCD86 significantly enhanced class I cytotoxicity and CD8 + T cell dependent CTL responses.