论文部分内容阅读
[目的]了解耐药鲍曼不动杆菌(Acinetobacter baumannii,Ab)中耐药基因的分布情况,并比较基于耐药基因分型和脉冲场凝胶电泳分型的差异性。[方法]对2005年宁波市第二医院收集的27株流行和散发的Ab进行耐药分析,并对脉冲场凝胶电泳方法和基于耐药基因聚合酶链反应的聚类分析方法进行比较。[结果]27株Ab耐多种抗生素和携带多种耐药基因。基于多个类别的耐药基因分型与脉冲场凝胶电泳方法分型相比有88.9%的一致性,而且耐药基因分型能更好地反映不同耐药株的耐药过程。[结论]与脉冲场凝胶电泳方法相比,耐药基因分型方法可以用于院内感染的流行研究,且有助于临床的诊断和治疗。
[Objective] To investigate the distribution of drug resistance genes in Acinetobacter baumannii (Ab) and to compare the differences based on resistance genotyping and pulsed-field gel electrophoresis (PAGE). [Methods] Twenty-seven isolates of Ab, collected from the Second Hospital of Ningbo City in 2005, were analyzed for drug resistance. Pulsed-field gel electrophoresis and cluster analysis based on polymerase chain reaction-resistant genes were compared. [Result] 27 strains of Ab were resistant to many kinds of antibiotics and carrying a variety of drug resistance genes. Resistance genotyping based on multiple categories was 88.9% more consistent with the pulsed-field gel electrophoresis and the resistant genotyping was better able to reflect the resistance of different drug-resistant strains. [Conclusion] Compared with pulsed-field gel electrophoresis, the drug resistance genotyping method can be used for the epidemiological study of nosocomial infections and is helpful for clinical diagnosis and treatment.