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运用电镜放射自显影术探讨了可溶性浓缩铀UO_2F_2在机体内亚细胞水平的蓄积动态。发现浓缩铀UO_2F_2在内污染危害早期集中于主要沉积器官肾脏,尤其呈选择性沉积于近曲细管上皮细胞核中和胞质的线粒体内,以及曲细尿管基底膜处,从而可导致近曲细管上皮细胞的变性、坏死和脱落。对肝组织细胞的电镜放射自显影观察表明,浓缩铀UO_2F_2开始主要沉积到肝细胞核中以及胞浆中可溶性蛋白质部位,随着观察时间的延长,浓缩铀UO_2F_2主要定位于肝细胞的线粒体上,其次是溶酶体中。浓缩铀UO_2F_2在骨组织细胞中的沉积是持续增升的,主要沉积在松质骨部位的骨细胞核和破骨细胞核中,而在胞质中,尤其是线粒体中,也有浓集的放射自显影径迹颗粒呈现,且其滞留期长,很难排除。
Electron microscopic autoradiography was used to investigate the dynamic accumulation of soluble uranium (UO 2 U 2) in the body at subcellular level. It was found that UO 2 F 2 enriched uranium was concentrated in the kidney of the major sedimentary organs, especially in the mitochondria of the nucleus and cytoplasm, and in the basement membrane of the kytoplasm, Tubular epithelial cells degeneration, necrosis and shedding. Electron microscope autoradiography of liver cells showed that uranium enriched uranium (UO 2 F 2) mainly deposited in the nucleus of hepatocytes and soluble protein in cytoplasm. With the extension of observation time, uranium enriched uranium (UO 2 F 2) mainly located on the mitochondria of hepatocytes, followed by Is lysosomes. The deposition of uranium enriched uranium (UO 2 F 2) in bone tissue cells is continuously increasing, mainly in the nuclei of osteoclasts and osteoclasts in the cancellous bone sites, whereas in the cytoplasm, especially in mitochondria, there is also a concentration of autoradiography Track particles appear, and its long residence time, it is difficult to rule out.