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目的 检测可诱导共刺激分子(inducibleco stimulator,ICOS)及相关免疫分子在SLE病人外周血T细胞亚群的表达,探索其与SLE疾病活动程度、病程及血清抗dsDNA抗体和免疫球蛋白含量间的关系,为进一步研究ICOS在SLE免疫病理中的作用奠定基础。方法 采用流式细胞术检测第二军医大学附属长海医院住院和门诊SLE患者 (n=51)以及健康体检者 (n=30)外周血T细胞亚群表面ICOS及其他免疫分子CD45RO、CD45RA、HLA DR的表达水平,观察ICOS在不同细胞亚群表达水平与SLE活动程度、病程、血清抗dsDNA抗体和血清免疫球蛋白含量间的关系。结果 与正常人相比,活动期及稳定期SLE患者外周血CD4+T细胞、CD8+T细胞表达ICOS的水平显著升高,但活动期患者与稳定期患者之间无统计学差异(P≥0 05);同一SLE患者在疾病活动期,外周血CD4+、CD8+、CD45RO+、CD4+CD45RO+和CD8+CD45RO+细胞表面ICOS的表达水平明显高于该患者经过治疗疾病缓解阶段(P<0 05);初发SLE患者CD45RO+细胞表达ICOS水平明显高于复发患者 (P<0 05);血清抗dsDNA抗体( +)及免疫球蛋白(IgG、IgM和IgA中任何一种 )异常升高的患者外周血CD45RO+及CD4+CD45RO+细胞表达ICOS的水平分别明显高于血清抗dsDNA抗体 ( -)和免疫球蛋白含量正常的患者(P<0 05)。结论 SLE患者外周血某些T
Objective To detect the expression of inducible co stimulator (ICOS) and related immune molecules in T lymphocyte subsets of peripheral blood in patients with SLE and to explore their relationship with the activity of SLE disease, the duration of disease and serum anti-dsDNA antibody and immunoglobulin content Relationship, to lay the foundation for the further study of the role of ICOS in SLE immunopathology. Methods Flow cytometry was used to detect ICOS and other immune molecules CD45RO, CD45RA and HLA-DR in peripheral blood of peripheral blood of hospitalized and outpatient SLE patients (n = 51) and healthy subjects (n = 30) of Changhai Hospital affiliated to Second Military Medical University DR expression levels were observed ICOS expression in different cell subpopulations and the degree of SLE activity, duration, serum anti-dsDNA antibody and serum immunoglobulin content relationship. Results The levels of ICOS in peripheral blood CD4 + T cells and CD8 + T cells in active and stable SLE patients were significantly higher than those in normal controls, but there was no significant difference between active patients and stable patients (P> The expression of ICOS on peripheral blood CD4 +, CD8 +, CD45RO +, CD4 + CD45RO + and CD8 + CD45RO + cells in the same SLE patients was significantly higher than that in the control group (P <0.05). The levels of ICOS in CD45RO + cells in newly diagnosed SLE patients were significantly higher than those in relapsed patients (P <0.05). Serum anti-dsDNA antibody (+) and patients with abnormally elevated immunoglobulin (IgG, IgM and IgA) The level of ICOS expression in CD45RO + and CD4 + CD45RO + cells was significantly higher than that in serum anti-dsDNA antibody (-) and immunoglobulin levels (P <0.05). Conclusion SLE patients with some of the peripheral blood T