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皮肤黑色素瘤在全世界的发生率和死亡率不断增加。对黑色素瘤的生物学研究,是目前的一个重要研究领域。最近,转基因鼠的技术突破,导致了可以建立鼠皮肤黑色素瘤模型的可能。目前,还没有对皮下黑色素瘤的生长进行定量研究的报告。我们首次应用一种被称为超声背向散射显微镜(UBM)的影像学方法,以轴向30μm和横向60μm的空间分辨率对鼠早期黑色素进行影像研究。从最早期的检测,鼠的B16F10黑色素瘤已经成像,直到几天后,它们的径长到2-5mm。用UBM测量黑色素瘤与肿瘤组织的病理检查有极好的吻合性。用UBM测量的黑色素瘤的高、宽相对的rms的误差分别是8.7％和4.2％,早期黑色素瘤的平均增加是0.37±0.06mm/天。影像图的三维显示获得黑色素瘤的体积,由计算机测得其大小,并与肿瘤进行比较。用假设的肿瘤的椭圆模型,用UBM测量的体积的相对误差小于17％。这些结果表明,用鼠的皮肤模型进行黑色素瘤的生物学研究是有益的,有助于用高频超声图像进行皮肤黑色素瘤的检测。
The incidence and mortality of skin melanoma are increasing worldwide. Biology research on melanoma is currently an important research area. Recently, technological breakthroughs in transgenic mice have led to the possibility of establishing murine skin melanoma models. At present, there has been no report of quantitative studies on the growth of subcutaneous melanoma. For the first time, we applied an imaging method called Ultrasound Backscatter Microscopy (UBM) to perform imaging studies on early melanin in the mouse with spatial resolution of 30 μm in the axial direction and 60 μm in the transverse direction. From the earliest tests, murine B16F10 melanomas have been imaged until after a few days, their diameters are 2-5mm. Measured by UBM melanoma and tumor tissue pathological examination has an excellent agreement. The errors of high and wide relative rms of melanoma measured by UBM were 8.7% and 4.2%, respectively, and the average increase of early melanoma was 0.37±0.06 mm/day. The three-dimensional display of the image shows the volume of the melanoma, which is measured by a computer and compared with the tumor. Using the hypothetical elliptical model of the tumor, the relative error of the volume measured with UBM was less than 17%. These results suggest that the use of murine skin models for the biological study of melanoma is beneficial, which helps to detect skin melanoma using high-frequency ultrasound images.