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目的 研究颈部迷走神经干刺激 (VNS)抑制癫痫发作上传通路过程中的关键核团及相关脑区。 方法 利用红藻氨酸 (KA)诱发大鼠复杂部分性癫痫发作 ,并结合Fos免疫组织化学方法观察左颈部迷走神经干电刺激后全脑及延髓内Fos的分布及电刺激的影响。 结果 VNS后脑干双侧孤束核、蓝斑、臂旁核、中脑导水管周围灰质有很强的特异性Fos表达 ,外侧缰核、丘脑室旁核、菱形核、下丘脑室旁核、杏仁中央核、终纹床核、隔外侧核、梨状皮质等脑区亦可见Fos阳性细胞。预先给予电刺激后海马、齿状回、额、顶、颞皮质区域Fos表达明显受到抑制。 结论 VNS后Fos阳性的脑区及核团可能是电刺激发挥抑痫作用的关键部位 ,其神经元活性的改变或递质调节可能间接或直接影响大脑皮质的功能
Objective To investigate the effects of vagus nerve stimulation (VNS) on the key nuclei and related brain regions in the process of epileptic seizure up-regulation. Methods KA was used to induce complicated partial seizures in rats. Fos immunohistochemistry was used to observe the distribution of Fos in whole brain and medulla oblongata and the effect of electrical stimulation on left vagus nerve in rats. Results There were strong specific Fos expression in nucleus tractus solitarius, locus coeruleus, parabrachial nucleus and periaqueductal gray of VNS after brainstem. The lateral habenular nucleus, paraventricular nucleus of thalamus, rhombohedral nucleus, paraventricular nucleus of hypothalamus , Almond central nucleus, striated nucleus, septal lateral nucleus, piriform cortex and other brain areas also visible Fos positive cells. Fos expression was significantly inhibited in hippocampus, dentate gyrus, frontal, parietal and temporal cortex after electrical stimulation. Conclusions Fos positive brain regions and nuclei after VNS may be the key part of electrical stimulation to exert antiepileptic effect. The changes of neuronal activity or the regulation of neurotransmitters may directly or indirectly affect the function of cerebral cortex