论文部分内容阅读
对３２ 例过敏性哮喘患者、２０ 名正常对照者， 分别用ＥＬＩＳＡ、ＲＴ－ ＰＣＲ、细胞培养技术，探讨哮喘患者白细胞介素－ １０（ＩＬ－ １０） 的变化。结果发现， 哮喘患者血浆和细胞培养上清液的ＩＬ－ １０ 值比正常对照组明显降低（ Ｐ＜ ０ ．０１） ，ＲＴ－ ＰＣＲ 也显示出正常对照组的扩增条带明显强于哮喘组。本研究说明， 哮喘患者外周血淋巴细胞的ＩＬ－ １０ 基因表达和血浆中ＩＬ－ １０ 水平均明显减少， 可能与哮喘的发生发展有关。
Twenty - two patients with allergic asthma and 20 normal controls were enrolled in this study. The changes of interleukin - 10 (IL - 10) in asthmatic patients were detected by ELISA, RT - PCR and cell culture respectively. The results showed that IL-10 levels in plasma and cell culture supernatant of asthmatic patients were significantly lower than those in normal controls (P <0.01), and RT-PCR also showed that the amplified bands in normal control group were significantly stronger than those in asthma group . This study shows that asthma patients with peripheral blood lymphocytes IL-10 gene expression and plasma IL-10 levels were significantly reduced, may be related to the occurrence and development of asthma.