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目的:研究人疱疹病毒6型(human herpesvirus-6,HHV-6)感染与神经胶质瘤的关系。方法:巢式PCR法检测40例神经胶质瘤样本和13例正常脑组织样本中HHV-6、人巨细胞病毒(human cytomegalovirus,HCMV)、HHV-7 DNA序列。免疫组化染色法检测神经胶质瘤样本和正常脑组织样本中HHV-6、HCMV、HHV-7抗原的表达。结果:巢式PCR法检测结果显示,神经胶质瘤组织HHV-6 DNA阳性率为42.5%,正常脑组织HHV-6 DNA阳性率为7.7%(P=0.020),神经胶质瘤组织HCMV、HHV-7DNA阳性率分别为20.0%和5.0%,正常脑组织未检测到HCMV和HHV-7 DNA(P值分别为0.087和0.566)。进一步用免疫组化染色法检测HHV-6早期抗原p41的表达,神经胶质瘤组织阳性率为27.5%,正常脑组织未见阳性表达(P=0.030)。同时检测HHV-6晚期抗原gp116/64/54的表达,神经胶质瘤组织阳性率为32.5%,正常脑组织未见阳性表达(P=0.014)。用HCMV pp65抗体进行免疫组化染色后发现其在神经胶质瘤中阳性率为12.5%,正常脑组织未见阳性表达(P=0.229)。HHV-7 pp85抗原在神经胶质瘤样本和正常脑组织样本中均未检出阳性表达。结论:根据PCR和免疫组化的检测结果,HHV-6感染在神经胶质瘤和正常脑组织中具有显著性差异,HHV-6感染在神经胶质瘤的病因和发展过程中起了一定作用。
Objective: To study the relationship between human herpesvirus-6 (HHV-6) infection and glioma. Methods: Nested PCR was used to detect the HHV-6, HCMV and HHV-7 DNA sequences in 40 glioma samples and 13 normal brain tissue samples. Immunohistochemical staining was used to detect the expression of HHV-6, HCMV and HHV-7 antigen in glioma samples and normal brain samples. Results: The results of nested PCR showed that the positive rate of HHV-6 DNA in glioma tissues was 42.5%, the positive rate of HHV-6 DNA in normal brain tissues was 7.7% (P = 0.020), the expression of HCMV, The positive rates of HHV-7 DNA were 20.0% and 5.0%, respectively. HCMV and HHV-7 DNA were not detected in normal brain tissue (P values were 0.087 and 0.566, respectively). The expression of early antigen HHV-6, p41 was detected by immunohistochemical staining. The positive rate of glioma was 27.5% and no expression was found in normal brain (P = 0.030). At the same time, the expression of gp116 / 64/54, a HHV-6 late antigen, was detected. The positive rate of glioma was 32.5% and no expression was found in normal brain (P = 0.014). Immunohistochemical staining with HCMV pp65 antibody showed that the positive rate was 12.5% in glioma and no positive expression in normal brain tissue (P = 0.229). No positive expression of HHV-7 pp85 antigen was detected in both glioma and normal brain samples. Conclusion: According to the results of PCR and immunohistochemistry, HHV-6 infection has significant difference between glioma and normal brain tissue. HHV-6 infection played a role in the pathogenesis and development of glioma .