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本文从顶体蛋白酶、顶体反应和体外受精三个方面研究了获能前就使用不同浓度的氯化铵对小鼠附睾精子的影响,精子经加有1mM、3mM、10mM和50mM氯化铵的孵育液孵育1小时后,用明胶底物膜法测定顶体蛋白酶活性,孵育2小时后制作电镜标本,观察顶体反应的发生情况,并用体外受精的方法检测精子的受精能力。结果,NH_4Cl在高浓度(≥10mM)时,能抑制小鼠精子的顶体蛋白酶活性和顶体反应,且药物浓度越高,抑制越明显,当NH_4Cl浓度为50mM时才对精子的体外受精能力产生抑制作用。提示从抑制溶酶体内容物释放方面寻找抑制顶体反应的药物作为避孕的新途径是切实可行的。
In this paper, the effects of different concentrations of ammonium chloride on the epididymal sperm of mice were studied before acrogenesis, from three aspects of acrosome protease, acrosome reaction and in vitro fertilization. Sperms were treated with 1mM, 3mM, 10mM and 50mM ammonium chloride After incubation for 1 hour, the acrosin activity was measured by the gelatin substrate membrane method. After 2 hours of incubation, the electron microscope was used to observe the occurrence of acrosome reaction and the sperm fertilization ability was tested by in vitro fertilization. As a result, NH_4Cl inhibited the acrosin activity and acrosome reaction of mouse sperm at high concentration (≥10mM), and the higher the drug concentration, the more obvious the inhibition. When NH_4Cl concentration was 50mM, the in vitro fertilization ability of sperm Produce inhibition. It is feasible to find a new way to prevent acrosome reaction from inhibiting the release of lysosomal contents.