论文部分内容阅读
目的观察汉黄芩素对慢性脑缺血大鼠的行为学影响,并探讨其可能的机制。方法制备慢性脑缺血模型(2-VO),术后6周分别给予汉黄芩素(实验组)和PBS(对照组)干预。2周后行Morris水迷宫评价大鼠的空间学习记忆功能变化,同时从缺血区脑组织的血管密度、缺血区脑细胞增殖等方面探讨其可能的机制。结果干预组大鼠的空间学习记忆能力较对照组明显改善,缺血区脑组织血管密度较对照组明显增高(P<0.05);而缺血区神经细胞的凋亡数量明显减轻(P<0.05)。结论汉黄芩素可以显著改善慢性脑缺血大鼠的空间学习记忆能力,其可能的机制与增加血管密度、减轻神经细胞凋亡有关的神经血管保护作用,其在缺血性脑血管病的治疗中有很大的应用前景。
Objective To observe the effect of wogonin on the behavior of rats with chronic cerebral ischemia and to explore its possible mechanism. Methods Chronic cerebral ischemia model (2-VO) was prepared. Wogonin (experimental group) and PBS (control group) were given at 6 weeks after operation. Morris water maze was used to evaluate the changes of spatial learning and memory in rats after 2 weeks. At the same time, its possible mechanism was explored from the cerebral density of ischemic brain tissue and the proliferation of ischemic brain cells. Results Compared with the control group, the spatial learning and memory abilities of rats in the intervention group were significantly improved. The density of blood vessels in the ischemic area was significantly higher than that in the control group (P <0.05), while the number of apoptotic neurons in the ischemic area was significantly reduced (P <0.05 ). Conclusion Wogonin can significantly improve the spatial learning and memory abilities in rats with chronic cerebral ischemia, and its possible mechanism is related to increasing the density of blood vessels and reducing the neurovascular protective effect of neuronal apoptosis. In the treatment of ischemic cerebrovascular disease, In the great application prospects.