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本文报告以中和试验做对照应用亲和素—生物素酶复合物—间接免疫组化法(ABC—IPA法)检测57倒临床诊断为病毒性脑膜炎患者脑脊液及血清中柯萨奇B组病毒(CBV)特异性IgM,检测的57例脑脊液及血清标本在发病早期(2～6天)CBV特异性IgM的阳性率分别为35.1％,15.8％。结果表明对中枢神经系统病毒感染的早期诊断,应用ABC—IPA法检测脑脊液中特异性IgM比血清中更有意义。
This article reports the neutralization test as control application of avidin - biotin enzyme complex - indirect immunohistochemistry (ABC-IPA method) detection 57 down clinical cerebrospinal fluid and serum Coxsackie B patients with viral meningitis The positive rate of CBV-specific IgM in 57 CSF and serum samples from early onset (2 to 6 days) was 35.1% and 15.8%, respectively. The results show that the early diagnosis of CNS virus infection, the application of ABC-IPA method for detection of specific IgM in cerebrospinal fluid than serum more meaningful.