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目的探讨人类白细胞抗原(HLA-E)蛋白在宫颈癌、宫颈高级别鳞状上皮内病变(HSIL)中的表达,及其与宫颈癌临床病理特点的关系。方法采用免疫组织化学染色检测HLA-E蛋白在18例HSIL病变宫颈组织、74例宫颈癌癌灶及其对应的20例癌旁正常宫颈组织中的表达,并评价其表达水平与宫颈癌的临床病理特点(临床分期、组织学类型、肿瘤分化程度、肿瘤直径大小、淋巴结转移、肌层浸润深度)的相关性。结果免疫组织化学染色结果显示,HLA-E蛋白在HSIL及宫颈癌患者病变组织中的表达水平明显高于癌旁正常宫颈组织(P=0.00),HLA-E蛋白在宫颈癌患者中的表达亦高于HSIL患者(P<0.01)。HLA-E蛋白的表达水平与宫颈癌的临床分期、肿瘤直径大小以及肌层浸润深度密切相关(P均<0.05),而与组织学类型、组织分化程度及淋巴结转移与否无关(P均>0.05)。结论高表达的HLA-E参与宫颈癌的免疫逃逸机制,通过改变患者的免疫状态可为宫颈癌的治疗提供新的方向和思路。
Objective To investigate the expression of human leukocyte antigen (HLA-E) in cervical cancer and cervical high-grade squamous intraepithelial lesion (HSIL) and its relationship with the clinicopathological features of cervical cancer. Methods Immunohistochemical staining was used to detect the expression of HLA-E protein in 18 cases of cervical HSIL lesions, 74 cases of cervical cancer and its corresponding normal tissues of 20 cases of adjacent normal tissues. The expression of HLA- Pathological features (clinical stage, histological type, degree of tumor differentiation, tumor diameter size, lymph node metastasis, depth of myometrial invasion). Results Immunohistochemical staining showed that the expression of HLA-E protein in HSIL and cervical cancer patients was significantly higher than that in adjacent normal cervical tissues (P = 0.00). The expression of HLA-E protein in cervical cancer patients Higher than HSIL patients (P <0.01). The expression of HLA-E protein was closely related to the clinical stage, the diameter of tumor and the depth of myometrial invasion (all P <0.05), but not to histological type, histological differentiation and lymph node metastasis (P> 0.05). Conclusion The high expression of HLA-E is involved in the immune escape mechanism of cervical cancer. It can provide new directions and ideas for the treatment of cervical cancer by changing the immune status of patients.